Responsible Professor: A.-M. Psarra  


Lecturers: A.-M. Psarra, P. Giannouli, K. Liadaki, N. Balatsos, V. Skamnaki, D. Stagos, D. Tsikou   

Recent advances in basic science and especially in biochemistry and molecular biology were based in the development of new methodologies and techniques. This course introduces basic and advanced experimental techniques that are broadly used for the structural and functional characterization of chemical biomolecules. The primary goal is the understanding of the theoretical background and application of many methods that are applied in biochemistry, biotechnology, and in cellular and molecular biology, but most importantly the training on the design and execution of experimental protocols. Based on oral presentations the students are practicing on the design of experimental protocols to decipher certain research subjects of biochemical and molecular proposals. 

Analytical description of the course  

• PCR  Methodologies  (D. Tsikou)
• Design of protein-expression vectors generation (V. Skamnaki)
• Spectrophotometry–Centrifugation (A.-M. Psarra)
• Immunochemical methods of analysis (A.-M. Psarra)
• Chromatographic methods of protein analysis (V. Skamnaki)
• Protein Electrophoresis and Western blotting (N. Balatsos)
• Methods of DNA, RNA electrophoresis, blotting and hybridization (N. Balatsos)
• Circular dichroism, dynamic light scattering and NMR analysis of biomolecules (D. Leonidas)
• Principles of crystallographic analysis of biomolecules (D. Leonidas)
• Flow cytometry-Principle and applications (K. Liadaki)
• Confocal microscopy-Principle and applications (A.-M. Psarra)
• Microarrays-Principle and applications (D. Stagos)
• Mechanical and thermal analysis of biopolymers (P. Giannouli)
• Methods of characterization of biomolecules interactions (A.-M. Psarra)
• Experimental protocols design (A.-M. Psarra)